简单易用，8-羟基脱氧鸟苷(8-OHdG)定量试剂盒

奋斗的小鸟

活性氧(ROS)自由基可以直接攻击生物大分子DNA诱发DNA氧化损伤。在各种氧化损伤中，鸟苷是DNA核碱基中最易被氧化的碱基，羟基自由基和超氧阴离子会直接攻击DNA分子中鸟嘌呤碱基的第8位碳原子，从而生成氧化性加合物8-OhdG（8-羟基脱氧鸟 苷）。


8-OHdG现已被广泛地应用在实验研究和临床诊断中，参与氧化应激与病变的指标检测与机理分析，对心血管疾病、糖尿病、神经退行性疾病（帕金森病，阿尔茨海默氏症）、以及癌症的临床诊疗都具有极大的应用价值。目前市场上主流使用的是竞争ELISA方法，因为它们不够精确，也不便于应用于细胞/组织的8-OHdG检测，更不能直接检测从细胞或组织分离的完整DNA。   
为此，艾美捷科技作为专业的生命科学领域解决方案供应商，为您推荐全球最新的8-OHdG直接定量试剂盒。其检测准确度与HPLC或LC-MS分析高度相关和接近。   
8-羟基脱氧鸟苷试剂盒原理
DNA包被特殊处理带有高DNA亲和性的微孔板上，8-OHdG通过捕获抗体和检测抗体来检测.通过酶标仪可以检测结果.大量得8-OHdG与光密度值成比例.  


<8-OHdG试剂盒实验流程，标准曲线，以及与液相质谱分析对比>
8-OHdG试剂盒优势：
1、比色实验简单易用，方便快速，整个试验完成需要3小时45分钟；
2、高灵敏度，检测8-OHdG极限低至2pg；
3、高特异性，只能检测8-OHdG，不能检测8-OHdG类似物；
4、利用完整DNA直接检测8-OHdG，能消除高分子量化合物的影响，例如：糖类和蛋白质等能竞争8-OHdG；
5、检测结果精确，近似接近于HPLC和LC-MS；
6、高度方便直接使用来自细胞，组织中的DNA，不需要DNA消化或者水解；
7、包含通用的阳性对照和阴性对照，能适用于定量任何种系8-OHdG；
8、本试剂盒实验结果更精确，灵敏，可靠，可靠并能显著降低背景信号.
《8-OHdG试剂盒已发表文章》
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      艾美捷科技与国内外优秀的生物试剂供应商保持着密切的合作关系，目前已成为众多国际知名品牌的中国总代理或一级代理，主要包括：Abbkine、Merck Millipore、Origene、AAT Bioquest、Cayman Chemical、Abnova 、Biovision、ProSpec、Hycult Biotech 、Equitech-Bio、Trevigen、Alomone Labs、Epigentek、ImmunoReagents 、Jackson、SouthernBiotech、US Biological 、Caisson Labs等，可以在第一时间为用户提供最前沿的专业资讯、完备的产品及物流服务。  

原文地址：https://zhuanlan.zhihu.com/p/54638113